Bob Hoskins
Eddie Valiant
Christopher Lloyd
Judge Doom
Charles Fleischer
Roger Rabbit/Benny The Cab/Greasy/Psycho (voice)
Kathleen Turner
Jessica Rabbit (voice)
Richard LeParmentier
Lt. Santino
Lou Hirsch
Baby Herman (voice)
Betsy Brantley
Jessica's Performance Model
Mel Blanc
Bugs Bunny/Daffy Duck/Tweety Bird (voice)
Mae Questel
Betty Boop (voice)
Richard Williams
Droopy (voice)
Wayne Allwine
Mickey Mouse (voice)
Frank Welker
Dumbo (voice)
Mickie McGowan
Mooing Cow/Flying Witch (voice)
Jack Angel
Additional Voices (voice)
Nancy Cartwright
Dipped Shoe (voice)
June Foray
Wheezy/Lena Hyena (voice)
Amy Irving
Jessica Rabbit (singing voice)
Edwin Craig
Arthritic Cowboy
Morgan Deare
Editor / Gorilla (voice)
Christopher Hollosy
Kid #2
Laura Frances
Blonde Starlet
Billy J. Mitchell
Forensic #2
James O'Connell
Conductor
Eugene Gutierrez
Teddy Valiant
April Winchell
Mrs. Herman (voice)
Tony Anselmo
Donald Duck (voice)
Mary T. Radford
Hippo (voice)
Joe Alaskey
Yosemite Sam / Foghorn Leghorn (voice)
David L. Lander
Smart Ass (voice)
Fred Newman
Stupid (voice)
Russi Taylor
Birds / Minnie Mouse (voice)
Les Perkins
Mr. Toad (voice)
Richard Williams
Droopy Dog
Tony Pope
Goofy / Big Bad Wolf (voice)
Peter Westy
Pinocchio (voice)
Cherry Davis
Woody Woodpecker