Frank Welker
Scooby-Doo / Fred Jones / Bikers (voice)
Mindy Cohn
Velma Dinkley (voice)
Grey DeLisle
Daphne Blake (voice)
Matthew Lillard
Shaggy Rogers / Shaky Joe (voice)
Fred Willard
Mr. Hubley (voice)
Finola Hughes
Professor Svankmajer (voice)
Gwendoline Yeo
Ms. Deitch (voice)
Matthew Gray Gubler
Winsor (voice)
Cathy Cavadini
Faith (voice)
Maulik Pancholy
Doctor (voice)
Michael Gough
Mr. Babbit / Blair / Grad Student #1 / Bikers (voice)
Kevin Michael Richardson
Tex / Cop #2 / Grad Student #3 / Bikers (voice)
John DiMaggio
Friz / GPS / Bikers (voice)
Dave Wittenberg
Policeman / Cop #1 / Grad Student #2 / Bikers (voice)